Bacterial Whole Genome Sequencing

Tender description

The University of Bristol is seeking a single provider to support a number of large research projects for which the research group undertakes surveillance of bacterial isolates from a variety of human and animal sources in the UK, Argentina and Thailand. The research is funded by for Arwain DGC (Defnydd Gwrthfioteg Cyfrifol / Leading on the responsible use of antimicrobials Wales); and the UKRI Biotechnology and Biological Sciences Research Council (BBSRC). General Requirements In order to fully characterise the genomes of Gram negative bacterial isolates for a variety of research projects, we require a bacterial whole genome sequencing specialist service on an Illumina platform generating 2x250bp paired-end reads at a minimum depth of coverage of 30x and a median insert sizes greater than 600bp. The bacteria under study are known to lose plasmids in cold storage conditions over time. In order to mitigate against plasmid loss we need a sequencing provider who has existing protocols that preserve the DNA of the isolates as close to the point of isolation as possible. For each isolate we can provide the equivalent of 8 OD600nm or 4 x 109 cells.