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Openclosing

RBGKEW1309 Long-Read Ash Genome Sequencing 2024

Published

Value

167,000 GBP

Description

Royal Botanic Gardens, Kew wishes to appoint a supplier to individually sequence the whole genomes of up to 300 European ash trees (Fraxinus excelsior) at a minimum of 15x whole genome coverage via a long-read technology. Fraxinus excelsior is a diploid and has a 1C genome size of 880 Mbp. In addition to the 300 individual ash trees, we wish to sequence a minimum of six technical replicates. Therefore, the total number of samples we wish to sequence is 306. RBG Kew intends to supply total genomic DNA for each sample with the following metrics: minimum of 10ug DNA; minimum concentration of 30ng/ml (as measured with a Quantus flurometer dsDNA assay); at least 50% of the sample in fragments with a minimum length of 40kb; minimum OD260/280 of 1.8; minimum OD260/230 of 1.8; minimum DIN of 7.

Timeline

Publish date

11 days ago

Close date

in 20 days

Buyer information

Royal Botanic Gardens Kew

Email:
procurement@kew.org

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Notice topic

Research services

Related topics

Marine research servicesResearch laboratory servicesResearch and experimental development services

Source

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